England manager Thomas Tuchel has omitted Josh King, James Garner and Jarrad Branthwaite from his squad to face world champions Spain in Madrid on Sunday night.
England travelled to Spain with a squad of 26 players but Uefa rules state that only 23 players can be selected in a matchday squad for Nations League games
The return to fitness of Ezri Konsa and Djed Spence - who missed Thursday's win over Croatia - has been a contributing factor in the trio's omission.
Arsenal defender Konsa could come straight back into the starting XI while James Trafford will replace Jordan Pickford in goal.
Garner replaced Declan Rice in the 77th minute of the 2-1 victory over Croatia at Wembley, scoring a goal that was subsequently disallowed, while Everton team-mate Branthwaite and Fulham's King were unused substitutes.
King received his first senior call-up for the two final Nations League fixtures in Group A3, but the midfielder, 19, will not feature in this international window.
However, fellow first-time call-ups Jobe Bellingham and James Hill are included.
Arsenal defender Ben White, who was booed when he came on as a substitute against Croatia, is also part of the reduced 23-man party.
England sit three points behind Spain in their group and know that a win will secure top spot in the table, as they…